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Abstract Communities are vital sources of support during crisis, providing collective contexts for shared identity and solidarity that predict supportive, prosocial responses. The COVID‐19 pandemic has presented a global health c...
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Abstract Communities are vital sources of support during crisis, providing collective contexts for shared identity and solidarity that predict supportive, prosocial responses. The COVID‐19 pandemic has presented a global health crisis capable of exerting a heavy toll on the mental health of community members while inducing unwelcome levels of social disconnection. Simultaneously, lockdown restrictions have forced vulnerable community members to depend upon the support of fellow residents. Fortunately, voluntary helping can be beneficial to the well‐being of the helper as well as the recipient, offering beneficial collective solutions. Using insights from social identity approaches to volunteering and disaster responses, this study explored whether the opportunity to engage in helping fellow community members may be both unifying and beneficial for those engaging in coordinated community helping. Survey data collected in the UK during June 2020 showed that coordinated community helping predicted the psychological bonding of community members by building a sense of community identification and unity during the pandemic, which predicted increased well‐being and reduced depression and anxiety. Implications for the promotion and support of voluntary helping initiatives in the context of longer‐term responses to the COVID‐19 pandemic are provided. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement.
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Attention is being given to healthcare initiatives with the potential to save money and improve lives. One example is social prescribing, which supports patients whose ill-health is exacerbated by loneliness. While evidence has ac...
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Attention is being given to healthcare initiatives with the potential to save money and improve lives. One example is social prescribing, which supports patients whose ill-health is exacerbated by loneliness. While evidence has accumulated attesting to social prescribing’s efficacy, one limitation has been the lack of a theoretical framework, which limits understanding of how tackling loneliness improves health. In our evaluation of a social prescribing pathway, we adopt a ‘Social Cure’ approach, which posits that social relations affect health. Our study will evaluate the efficacy of the pathway and determine the extent to which group processes are responsible for health improvements.
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Objectives The psychological impact of the prolonged lockdown measures in the UK as a response to the COVID-19 pandemic is unclear. Our aim was to determine if there are significant differences in self-control, self-efficacy, depr...
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Objectives The psychological impact of the prolonged lockdown measures in the UK as a response to the COVID-19 pandemic is unclear. Our aim was to determine if there are significant differences in self-control, self-efficacy, depressive symptoms and leisure motivation between UK older adults with differing levels of physical activity, and which of these variables can be used to predict activity level after 1 year of lockdown restrictions, Methods 521 adults aged 50-92 years completed an online survey consisting of several validated measures relating to physical activity, self-control, self-efficacy, depressive symptoms, and leisure motivation. Participant's responses were grouped into active (>= 150minutes activity per week) and inactive (<150minutes activity per week). Data was analysed using ANOVA, Pearson's Correlation and Multiple Regression (forward stepwise). Results We found significant differences in self-efficacy, self-control, and depressive symptoms between physically active vs inactive subjects. High levels of self-control and self-efficacy were associated with higher levels of activity and fewer depressive symptoms. Self-control, amotivation, depressive symptoms and self-efficacy were predictors of physical activity level. Conclusion Psychological variables including self-control, self-efficacy, depressive symptoms and amotivation can be used to predict physical activity levels in UK middle-aged and older adults following 1 year of Covid restrictions.
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Ferritin is a protein that stores and releases iron to prevent diseases associated with iron dysregulation in plants, animals, and bacteria. The conversion between iron-loaded holo-ferritin and empty apo-ferritin is an important p...
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Ferritin is a protein that stores and releases iron to prevent diseases associated with iron dysregulation in plants, animals, and bacteria. The conversion between iron-loaded holo-ferritin and empty apo-ferritin is an important process for iron regulation. To date, studies of ferritin have used either ensemble measurements to quantify the characteristics of a large number of proteins or single-molecule approaches to interrogate labeled or modified proteins. Here we demonstrate the first real-time study of the dynamics of iron ion loading and biomineralization within a single, unlabeled ferritin protein. Using optical nanotweezers, we trapped single apo- and holo-ferritins indefinitely, distinguished one from the other, and monitored their structural dynamics in real time. The study presented here deepens the understanding of the iron uptake mechanism of ferritin proteins, which may lead to new therapeutics for iron-related diseases.
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Abstract A mobile remote standoff Raman spectroscopy system operational at typical distances of 10?m was developed specifically for research of historical sites and wall paintings recently. Here we present an upgrade to that syste...
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Abstract A mobile remote standoff Raman spectroscopy system operational at typical distances of 10?m was developed specifically for research of historical sites and wall paintings recently. Here we present an upgrade to that system informed by a thorough experimental investigation of the relevant laser-induced degradation issues. Reflectance spectroscopy as a more sensitive technique than Raman spectroscopy was used for monitoring and a new phenomenon of reversible alterations was detected in many paint samples at very low laser intensities of less than 1?W/cm2 when Raman measurements detected no changes. Contrary to conventional wisdom, the intensity threshold for safe operation was found to decrease significantly for larger incident irradiation area in the case of a vermilion oil paint sample. Damage threshold in intensity for each material needs to be determined for different spot sizes, which can be orders of magnitude lower for 1?mm spot size compared with micro-Raman. Results from this study is also relevant to portable Raman systems which use similarly large spot sizes. However, the larger spot size still generates more Raman photons overall under safe operation than micro-Raman systems. Continuous-wave (CW) lasers are found to be best suited to efficient, that is more Raman signal detected over a given measurement time, and safe Raman operation than ns-pulse lasers at the same wavelength. While the damage threshold in intensity for ns-pulse lasers is much higher than that of CW lasers, the pulse energy allowed in one pulse for safe operation is still too low to allow detection of Raman signal, and the need for multiple pulses makes pulse laser inefficient owing to the low repetition rate necessary to ensure adequate heat dissipation between pulses. The safety of the upgraded system was evaluated and found that no permanent laser-induced degradation was detected within 60?s of laser irradiation for any of the paint samples.
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Acute morning fasted exercise may create a greater negative 24-hr energy balance than the same exercise performed after a meal, but research exploring fasted evening exercise is limited. This study assessed the effects of 7-hr fas...
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Acute morning fasted exercise may create a greater negative 24-hr energy balance than the same exercise performed after a meal, but research exploring fasted evening exercise is limited. This study assessed the effects of 7-hr fasting before evening exercise on energy intake, metabolism, and performance. Sixteen healthy males and females (n= 8 each) completed two randomized, counterbalanced trials. Participants consumed a standardized breakfast (08:30) and lunch (11:30). Two hours before exercise (16:30), participants consumed a meal (543 +/- 86 kcal; FED) or remained fasted (FAST). Exercise involved 30-min cycling (similar to 60% VO2peak) and a 15-min performance test (similar to 85% VO2peak; 18:30). Ad libitum energy intake was assessed 15 min postexercise. Subjective appetite was measured throughout. Energy intake was 99 +/- 162 kcal greater postexercise (p < .05), but 443 +/- 128 kcal lower over the day (p < .001) in FAST. Appetite was elevated between the preexercise meal and ad libitum meal in FAST (p < .001), with no further differences (p >= .458). Fat oxidation was greater (+3.25 +/- 1.99 g), and carbohydrate oxidation was lower (-9.16 +/- 5.80 g) during exercise in FAST (p < .001). Exercise performance was 3.8% lower in FAST (153 +/- 57 kJ vs. 159 +/- 58 kJ, p < .05), with preexercise motivation, energy, readiness, and postexercise enjoyment also lower in FAST (p < .01). Fasted evening exercise reduced net energy intake and increased fat oxidation compared to exercise performed 2 hr after a meal. However, fasting also reduced voluntary performance, motivation, and exercise enjoyment. Future studies are needed to examine the long-term effects of this intervention as a weight management strategy.
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We describe a surface charge imaging method for heterogeneous biosilicas based on relationships between zeta (ζ) potential, feature size of nanoparticles, and PDMPO fluorescence and apply it to silicified structures from plants a...
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We describe a surface charge imaging method for heterogeneous biosilicas based on relationships between zeta (ζ) potential, feature size of nanoparticles, and PDMPO fluorescence and apply it to silicified structures from plants and diatoms. The methodology provides the first opportunity to map the surface charge of large heterogeneous biosilica materials and indicates that local surface charge is related to morphology below the diffraction limit (ca. 20–130 nm) with sharper features showing less negative zeta potential equivalent surface charge suggesting that the zeta potential of silica structures can be adjusted by engineering surface morphology. We show that the approach can be used to study living silicified biological tissues without recourse to sectioning and fixation. Further, the approach could be used for the study of other metal oxides possessing hydroxylated moieties. The method has potential to open up opportunities for the engineering of materials with defined charge characteristics for the solution of biomedical engineering problems including materials for tissue replacement.
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Abstract Type-2 diabetes (T2D) is characterised by a dysregulation of metabolism, including skeletal muscle insulin resistance, mitochondrial dysfunction, and oxidative stress. Reactive species, such as methylglyoxal (MGO) and 4-h...
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Abstract Type-2 diabetes (T2D) is characterised by a dysregulation of metabolism, including skeletal muscle insulin resistance, mitochondrial dysfunction, and oxidative stress. Reactive species, such as methylglyoxal (MGO) and 4-hydroxynonenal (4-HNE), positively associate with T2D disease severity and can directly interfere with insulin signalling and glucose uptake in skeletal muscle by modifying cellular proteins. The multifunctional dipeptide carnosine, and its rate-limiting precursor β-alanine, have recently been shown to improve glycaemic control in humans and rodents with diabetes. However, the precise mechanisms are unclear and research in human skeletal muscle is limited. Herein, we present novel findings in primary human T2D and lean healthy control (LHC) skeletal muscle cells. Cells were differentiated to myotubes, and treated with 10?mM carnosine, 10?mM β-alanine, or control for 4-days. T2D cells had reduced ATP-linked and maximal respiration compared with LHC cells (p?=?0.016 and p?=?0.005). Treatment with 10?mM carnosine significantly increased insulin-stimulated glucose uptake in T2D cells (p?=?0.047); with no effect in LHC cells. Insulin-stimulation increased MGO-modified proteins in T2D cells by 47%; treatment with carnosine attenuated this increase to 9.7% (p?=?0.011). There was no effect treatment on cell viability or expression of other proteins. These findings suggest that the beneficial effects of carnosine on glycaemic control may be explained by its scavenging actions in human skeletal muscle.
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The ownership of sex dolls has become an increasingly controversial social issue over the last five to ten years, with many in society (and academia) calling for the criminalization of such dolls. At the root of these calls is the...
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The ownership of sex dolls has become an increasingly controversial social issue over the last five to ten years, with many in society (and academia) calling for the criminalization of such dolls. At the root of these calls is the implicit (and often explicit) assumption that sex doll ownership contributes to increases in negative social attitudes toward women, and sexual offense risk among doll owners. However, there are yet to be any empirical examinations of these claims. In this work we compared the psychological characteristics and comparative sexual aggression proclivities of sex doll owners (n = 158) and a non-owner comparison group (n = 135). We found no substantive differences in most psychological traits. Doll owners scored lower than comparators in relation to sexual aggression proclivity. They were, however, more likely to see women as unknowable, the world as dangerous, and have lower sexual self-esteem. They also had more obsessive and emotionally stable personality styles. We conclude that there is no evidence that sex doll owners pose a greater sexual risk than a non-owning comparison group, before highlighting the need for more evidence-informed social debates about the use of sex dolls in modern society.
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The tumour suppressor p53 is a nuclear transcription factor with key roles during DNA damage to enable a variety of cellular responses including cell cycle arrest, apoptosis and DNA repair. JMY is an actin nucleator and DNA damage...
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The tumour suppressor p53 is a nuclear transcription factor with key roles during DNA damage to enable a variety of cellular responses including cell cycle arrest, apoptosis and DNA repair. JMY is an actin nucleator and DNA damage-responsive protein whose sub-cellular localisation is responsive to stress and during DNA damage JMY undergoes nuclear accumulation. To gain an understanding of the wider role for nuclear JMY in transcriptional regulation, we performed transcriptomics to identify JMY-mediated changes in gene expression during the DNA damage response. We show that JMY is required for effective regulation of key p53 target genes involved in DNA repair, including XPC, XRCC5 (Ku80) and TP53I3 (PIG3). Moreover, JMY depletion or knockout leads to increased DNA damage and nuclear JMY requires its Arp2/3-dependent actin nucleation function to promote the clearance of DNA lesions. In human patient samples a lack of JMY is associated with increased tumour mutation count and in cells results in reduced cell survival and increased sensitivity to DNA damage response kinase inhibition. Collectively, we demonstrate that JMY enables p53-dependent DNA repair under genotoxic stress and suggest a role for actin in JMY nuclear activity during the DNA damage response.
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